Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection
نویسندگان
چکیده
منابع مشابه
Serum-free large-scale transient transfection of CHO cells.
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies at scales beyond 10 L. Unfortunately, both approaches yield higher levels of recombinant protein (r-protein) in the presence of serum than in...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2009
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-009-9224-x